CD Skripsi

Seleksi Bakteri Lokal Penghasil Lipase Dari Tanah Tempat Pembuangan Sampah Kecamatan Tampan Pekanbaru

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ABSTRACT
Lipase is an enzyme that can hydrolyze fat into fatty acids and glycerol. Lipase enzymes are widely needed in the fields of health, industry and biotechnology, but their availability is still low and expensive. One alternative in producing lipase is using microorganisms, namely bacteria. Lipase-producing bacteria can be found in various habitats such as industrial waste, vegetable oil processing plants, dairy companies, soil contaminated with oil. The purpose of this study was to select lo-cal Riau bacterial isolates from soil isolation from landfills that were able to pro-duce lipase enzymes. Selection test for lipolytic ability of 11 bacterial isolates us-ing paper disc by measuring the clear zone formed on solid selective media by adding corn oil as a substrate. The results showed that 3 bacterial isolates had the largest clear zone in succession, namely BP1 2.21±0.58 cm with a lipolytic index of 1.58. BP6 isolate was 1.80±0.18 with a lipolytic index of 1.31 and BPT6 isolate was 1.67±0.07 cm with a lipolytic index of 1.32. The lipase activity test of the three isolates that had the largest clear zones, namely BP1, BP6 and BPT6 were tested using the titration method, the results of the lipase activity values sequen-tially, namely BP1 of 0.85 U /ml, BP6 of 1.37 U/ml and BPT6 of 1.52 U/ml at 24 hours incubation.
Keywords: bacteria, corn oil, landfill soil, lipase enzyme, lipase activity

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1603110504

Penerbit

Pekanbaru : Universitas Riau - FMIPA - Biologi., 2022

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1603110504

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Deti

Versi lain/terkait

Lampiran Berkas

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