CD Skripsi

Profil Metabolit Dan Aktivitas Antioksidan Fermentasi Ko-Kultur Isolat Jamur Lokal Riau Penicillium Sp. Dan Trichoderma Sp.

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Local isolates of the Riau fungus Penicillium sp. LBKURCC34, Trichoderma asperellum LBKURCC1 and Trichoderma asperelloides LBKURCC2 are well-known potential producers of enzymes and secondary metabolites with antimicrobial activity. Several studies have shown that these secondary metabolites have potential as antioxidants. Co-culture fermentation is known to increase the structural diversity of secondary metabolites compared to single fermentation. In this research, co-culture fermentations were done for Penicillium sp. LBKURCC34 and Trichoderma asperellum LBKURCC1 (Co-Culture A), Penicillium sp. LBKURCC34 and Trichoderma asperelloides LBKURCC2 (Co-Culture B) and the three fungi Penicillium sp. LBKURCC34, Trichoderma asperellum LBKURCC1 and Trichoderma asperelloides LBKURCC2 (Co-Culture C). The extraction was performed using organic solvents butanol (BuOH), ethyl acetate (EtOAc) and acetone (Ace). These extracts were analyzed using the LC/MS-MS to identify metabolite profiles and the antioxidant activity was analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-Azinobis-3-ethylbenzotiazoline-6-sulfonic acid (ABTS) methods. Metabolomic analysis showed that co-culture etil acetat extracts of these fungi contained N-(2-phenylethyl) acetamide and N-Acetyltyramine which are tyramids of the alkaloid group, phenolic compounds 2-aminophenol and 6-Methyl-2-pyridinemethanol, g-Butyrobetaine, maculosin which is acyclodipeptide group, (E, E)-alpha-Farnesene which is an acyclic sesquiterpene group and betaine. In addition, the most active antioxodant extract in both method, Butanol C, contains L-phenylalanine and g-Butyrobetaine compounds. The antioxidant activity of co-culture A, B and C were determined using the DPPH and ABTS method, obtained the IC50 values of all butanol dan etil acetate extracts of all co-cultures were active and very active while all aceton extracts from all co-cultures are not active. Furthermore, ABTS method showed a similar pattern to the DPPH method

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1803112344

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2022

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1803112344

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

nadya

Versi lain/terkait

Lampiran Berkas

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