CD Skripsi

Potensi Bacillus Spp. Dalam Menghasilkan Biosurfaktan Pada Substrat Minyak Pelumas

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ABSTRACT
Biosurfactants are extracellular macromolecules produced by microorganisms. Biosurfactants are physically environmentally friendly, and have low toxicity. This study aims to determine the potential of Bacillus spp. in producing biosurfactant compounds on lubricating oil substrates. The methods used are: drop collapse test, oil spreading technique, emulsification activity, characterization of biosurfactants using Thin Layer Chromatography (TLC) and surface tension test using a Tensiometer (CSC-DuNouy). Supernatant fermented from the three isolates of Bacillus spp. showed positive results for drop collapse test and oil spreading technique with the highest clear zone diameter indicated by Bacillus sp. isolate Sp 48 of 10.2 mm on used lubricating oil substrate and Bacillus sp. isolate Sp 34 of 7.3 mm on a new lubricating oil substrate. Bacillus sp. isolate Sp 48 showed the highest emulsification index value (E24) of 99.5% on used lubricating oil substrate and 75.3% on new lubricating oil substrate. Thin Layer Chromatography test results of the biosurfactants produced by the three isolates of Bacillus spp. is a group of lipopeptides with each Rf value: Bacillus sp. isolates Sp 48 (0.89), Bacillus sp. isolates Sp 34 and Bacillus sp. isolate Sp 84 (0.90). Biosurfactant produced by Bacillus sp. isolate Sp 48 can reduce the surface tension of 19.7 dyne/cm, Bacillus sp. isolate Sp 34 of 17.9 dyne/cm and Bacillus sp. isolate Sp 84 of 15.7 dyne/cm with a new lubricating oil carbon source compared to the control. Production of biosurfactants from the three isolates of Bacillus spp. using lubricating oil as a substrate developed in various industries.
Key words: Bacillus spp., biosurfactant, emulsification, lipopeptide, lubricating oil.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1803112455

Penerbit

Pekanbaru : Universitas Riau - FMIPA - Biologi., 2022

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1803112455

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Deti

Versi lain/terkait

Lampiran Berkas

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