CD Skripsi

Isolasi, Karakterisasi Dan Identifikasi Bakteri Pelarut Fosfat Asal Lahan Gambut Bengkalis Riau

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ABSTRACT
Peatland is a land that has a high organic matter content so that it is widely used by bacteria, one of which is phosphate solubilizing bacteria (PSB). PSB is a soil microbe capable of dissolving phosphate by excreting a number of organic acids such as oxalic, succinic, fumaric, malic and possessing phosphatase and phytase enzymes. This study aims to isolate, select and characterize the morphology, physiology, biochemistry and molecular identification by sequencing the 16s rRNA gene. Soil samples were taken at random and isolated by pour plate method using Pikovskaya selective agar. Phosphate dissolving activity was tested by looking at the formation of a clear zone around the colony and measuring the phosphate solubilization index. Eight isolates of phosphate solubilizing bacteria were successfully obtained from peat soil where two isolates were potential to dissolve phosphate and had the highest phosphate solubilization index, based on the results of 16S rRNA gene sequencing, the two bacterial isolates were identified as Burkholderia cepacia (GBS-1) and Burkholderia tropica (GBK-2) which have the highest phosphate solubilization index of 2.65 and 3.81.
Keywords: Clear zone, isolation, peatland, phosphate solubilizing bacteria.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1803111953

Penerbit

Pekanbaru : Universitas Riau - FMIPA - Biologi., 2022

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1803111953

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Deti

Versi lain/terkait

Lampiran Berkas

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