CD Skripsi

Isolasi Dan Uji Antioksidan Senyawa Metabolit Sekunder Ekstrak Etil Asetat Tumbuhan Paku Pyrrosia Longifolia

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SUMMARY
Pyrrosia longifolia is an epiphytic plant that belongs to the family Polypodiaceae. Other species, such as P. petiolosa and P. lancelota have been reported as sources of antioxidants and anti-inflammatory agents, however this species has not received as much attention as it acknowledges. The purpose of this research was to isolate secondary metabolites having antioxidant activity from an ethyl acetate extract of P. longifolia. The species was extracted employing the maceration method with methanol, and then fractionated with the liquid-liquid extraction technique with the n-hexane, dichloromethane, and ethyl acetate. Eight different fractions were obtained after using liquid vacuum chromatography to separate the ethyl acetate extract. In fraction 1, an impure green precipitate was produced, which then recrystallized. The sample code for the pure compound in the shape of a white solid was 20-05-C-F1. Analysing the purification of 20-05-C-F1 using HPLC and melting point 156-158°C. The compound was elucidated by using UV/vis, FTIR, and NMR spectroscopy techniques, and it was found as naringin based on the data comparison. Results of antioxidant activity test on n-hexane extract, dichloromethane, ethyl acetate, water, and the fraction of the ethyl acetate extract showed strong antioxidant activity because it had IC50 < 100 μg/mL and naringin compound was isolated from fraction 1 had an IC50 > 1000 μg/mL indicating very weak antioxidant activity.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1703113357

Penerbit

Pekanbaru : Universitas Riau - FMIPA - Kimia., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1703113357

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

TIAR

Versi lain/terkait

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