CD Skripsi

Isolasi Dan Uji Antijamur Senyawa Fenolik Dari Ekstrak Metanol Jamur Endofit Botryosphaeria Rhodina

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SUMMARY
Endophytes are microorganisms that live within plant tissue without causing damage to the host. Endophytic microorganism promotes plant growth and is well-known as a source of novel bioactive secondary metabolites which potential as a candidate of antimicrobial, antiinsects, anticancer and other properties. Botryosphaeria rhodina is an endophytic fungus isolated from the roots of mangrove Xylocarpus granatum J. Koenig in Muara Sungai Siput, Bengkalis Regency. The objective of this research were to isolate and identify secondary metabolites produced by B. rhodina and to evaluate their antifungal properties. In order to obtain methanol and n-hexane extracts from the fungus, the process of secondary metabolite extraction was performed in rice-based fermentation. Following this, ethyl acetate was used to partition the mixture. Using Vacuum Liquid Chromatography (VLC), the methanol extract was further separated into 16 fractions, which were then combined into seven primary fractions. Due to the presence of a precipitate in fraction I, it was necessary to recrystallize it with n-hexane before conducting Thin Layer Chromatography (TLC), melting point and HPLC analysis to determine the fraction's purity. The pure compound that was produced as a result was designated 21-07-EM-FI. The compound was analyzed by using UV-Vis and FT-IR spectrophotometry techniques and the results showed maximum absorption at 285 nm and 439 nm, and wave numbers 3474 cm-1, 2952 cm-1, 1592 cm-1, 1410 cm-1, 1369 cm-1 and 1264 cm-1, respectively. The minimum inhibitory concentration of 21-07-EM-FI against C. albicans, C. glabrata
and C. krusei was determined using the microdilution technique to be 50 μg/mL, 25 μg/mL and 25 μg/mL respectively, while the minimum fungicidal concentration
were > 100 μg/mL for C. albicans and 100 μg/mL for C. glabrata and C. krusei.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1903112228

Penerbit

Pekanbaru : Universitas Riau - FMIPA - Kimia., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1903112228

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

TIAR

Versi lain/terkait

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