CD Skripsi

Uji Potensi Flavonoid Dan Fenolik Dari Tumbuhan Paku Sayur (Diplazium Esculentum) Sebagai Inhibitor Enzim Α-Amilase Dan Α-Glukosidase

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SUMMARY
Diplazium esculentum is a species of fern belonging to the Athyriaceae family which can be used as a medicinal plant, one of which is suspected of being a potential inhibitor of α-amylase and α-glucosidase enzymes. This study aims to determine the relationship between the total flavonoids and phenolics content in the ethyl acetate and water extract of D. esculentum and their ability to act as inhibitors of α-amylase and α-glucosidase activities. Samples were extracted by maceration method using methanol solvent and followed by partitioned extract with two solvents namely n-hexane and ethyl acetate. Total flavonoid content was determined using the aluminium chloride colorimetric (AlCl3) method and total phenolics using the Folin-Ciocalteu method. IC50 of ethyl acetate and water extract on α-amylase activity was determined using the DNS method and the absorbance of the product was measured using a UV-Vis Spectrophotometer at λ 540 nm, while for α-glucosidase using p-NPG (p-nitrophenyl-α-D-glucopyranoside) method and the absorbance of the product was measured using a microplate reader at λ 405 nm. The results of this study indicated that there is a correlation between flavonoids and phenolics on the inhibition of α-amylase and α-glucosidase enzyme activity based on their IC50 values. The total flavonoids and phenolics content from ethyl acetate extract were higher than water extract with values of 149.90 ± 27.20 μg QE/g FW and 121.25 ± 8.29 μg GAE/g FW respectively. The best IC50 value for α-amylase was from water extract of 108.05 μg/mL, while for α-glucosidase was the ethyl acetate extract of 160.53 μg/mL. These data indicate that the fern species D. esculentum has the potential to act as an inhibitor of α-amylase and α-glucosidase although not as good as the acarbose positive control

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1803112755

Penerbit

Pekambaru : Universitas Riau - FMIPA - Kimia., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1803112755

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

puja

Versi lain/terkait

Lampiran Berkas

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