CD Skripsi
Aktivitas Antimikroba Dan Antioksidan Ekstrak Ko-Kultur Isolat Jamur Lokal Riau Penicillium Sp. Lbkurcc34 Dan Lbkurcc38
SUMMARY
Penicillium sp. LBKURCC34 and LBKURCC38 are fungi isolated from peat soil of the primary forest of the Giam Siak Kecil Bukit Batu Biosphere Reserve (GSKBB) of Riau Province. These fungi are known to have the potential to produce secondary metabolites that have antimicrobial and antioxidant activity. To know their pontency this research was conducted to produce secondary metabolites using co-culture batch fermentation between Penicillium sp. LBKURCC34 and LBKURCC38. Secondary metabolites extraction was carried out using ethyl acetate (EtOAc), butanol (BuOH) and acetone (Ace) solvents. These extracts were tested for their antimicrobial activity using the disc diffusion method, while the antioxidant activity was tested using the DPPH and ABTS methods. The results of the antimicrobial activity test showed that, based on the clear zone area, the three extracts from the co-culture of the fungus Penicillium sp. LBKURCC3438 at concentrations of 2000 and 200 μg/disc did not have antibacterial activity against the four pathogenic bacteria. At a concentration of 2000 μg/disc, only the EtOAc and BuOH extracts had antifungal activity against C. albicans, while at a concentration of 200 μg/disc the three extracts had antifungal activity against C. albicans. The antioxidant activity test results obtained were extracts that had antioxidant activity in counteracting DPPH and ABTS radicals, namely BuOH and EtOAc, while extracts that did not have antioxidant activity were Ace extracts. Based on the AAI value, the three extracts were categorized as weak with an index
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