CD Skripsi

Optimasi Suhu Dan Agitasi Produksi Enzim Amilase Dari Jamur Termofilik Aspergillus Sp. Lbkurcc304 Dalam Media Produksi Cair

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SUMMARY
Amylase is a group of enzymes that have the ability to break the glycoside bonds found in starch molecules. Amylase can be produced by several microbes such as Aspergillus sp. LBKURCC304 through a submerged fermentation process. This study aims to optimize the environmental conditions for the growth of Aspergillus sp. LBKURCC304 in produced amylase enzymes. The growth environmental conditions that were focused on in this study were temperature (40°C, 45°C, 50°C and 55°C) and agitation speed (50 rpm, 100 rpm, 150 rpm and 200 rpm). The activity of amylase from Aspergillus sp. was determined using the Nelson-Somogy method, determination of protein content using the Lowry method and the specific activity of the amylase enzyme from Aspergillus sp. was obtained by comparison the activity of the amylase enzyme with the protein content. The data from this study were statistically tested using the ANOVA and Duncan multiple range methods at a significant rate of 5%. The results of this study showed that the maximum amylase enzyme production by Aspergillus sp. was obtained at 40ºC and agitation rate of 50 rpm with an amylase enzyme activity of 0.4777 ± 0.0085 U/mL and specific activity of 1.0599 ± 0.0086 U/mL mg. The highest protein content at 150 rpm agitation was 0.8317 ± 0.0165 mg/mL.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1603110742

Penerbit

Pekanbaru : Universitas Riau - FMIPA - Kimia., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1603110742

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

PUJA

Versi lain/terkait

Lampiran Berkas

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