CD Skripsi

Isolasi Dan Identifikasi Bakteri Asam Laktat (Bal) Dari Makanan Tradisional Fermentasi Ikan Patin (Pangasius Hypophthalmus) Puduong Asal Kabupaten Kuantan Singingi

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SUMMARY
Puduong is a traditional fermented food from catfish originating from Kuantan Singingi Regency, which is almost extinct because it is not longer known, especially by the younger generation. However, this food is thought to contain lactic acid bacteria (LAB). A large number of fermented food usually contain LAB. Puduong is thought to contain LAB, which can act as a probiotic agent, which, when consumed, will have a good impact on the health of the body and produce metabolites that have the ability to inhibit the growth of harmful microorganisms in the body. This research aims to isolate and identify LAB from puduong. Isolation of LAB in this study was carried out using serial dilution 10-3 to 10-5 and inoculation using the spread plate method. Purification of LAB isolates was carried out by the 4-quadrant streak method. The purity of LAB isolates were identified using light microscopy. Morphological identification was carried out macroscopically (shape, edges, color, size, elevation of the colony) and microscopically (gram staining). Biochemical test through catalase test and carbohydrate fermentation test using four carbohydrates (d-glucose, fructose, lactose, sucrose). Physiological test through resistance tests to 6.5% NaCl salt levels and resistance tests to temperatures of 15°C and 45°C. The results showed that 42 isolates of LAB had characteristics of the genus Lactobacillus spp., Lactococcus spp. and Streptococcus spp. which were Gram positive, coccus/bacil cell shape, catalase-negative, homofermentative and able to reduce four types of sugars (d-glucose, fructose, lactose, and sucrose). 23 LAB isolates obtained could survive on media that have a NaCl salt content of 6.5%, 31 LAB isolates could survive at temperatures of 15°C and 45°C, 10 LAB isolates could only survive at 15°C and one other LAB isolate could only survive at 45°C.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1803112331

Penerbit

Pekanbaru : Universitas Riau - FMIPA - Kimia., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1803112331

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

PUJA

Versi lain/terkait

Lampiran Berkas

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