CD Skripsi

Aktivitas Ekstrak Kasar Enzim Kolagenase Dari Kulit Ikan Kepala Indonesia (Channa micropeltes) Pada pH Berbeda

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Collagenase enzyme is one of the protease enzymes that can degrade the polypeptide bonds of collagen when the protein has not been denatured. Collagenase enzymes are widely used in industrial applications both food and non-food and to be able to work optimally, collagenase enzymes have activities that can be influenced by substrates, temperature, inhibitors, and pH. This study aims to determine the effect of different pH on the activity of collagenase enzyme crude extract from toman fish skin (Channa micropeltes). This research method uses experiments, namely conducting direct experiments with a non-factorial complete randomized design (CRD) method with 3 replicates. The treatments used in this study were different pH, namely pH 7.5, pH 8.5, and pH 9.5. The parameters tested were collagenase enzyme crude extract, collagenase enzyme crude extract activity, and soluble protein concentration. The results showed the activity of collagenase enzyme crude extract from toman fish skin (Channa micropeltes) at different pH 7.5 was 0.46 U/mL, pH 8.5 was 0.65 U/mL, and pH 9.5 was 0.55 U/mL. The optimum pH of collagenase enzyme from Indonesian snakehead fish skin is pH 8.5. Increasing the pH value can increase the enzyme activity up to the optimum pH in the crude extract of collagenase enzyme from Indonesian snakehead fish skin (Channa micropeltes).

Keywords: Channa micropeltes, crude extract, collagenase enzyme, pH, proteins.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1904124868

Penerbit

Pekanbaru : Universitas Riau - FAPERIKA – Teknologi Hasil Perikanan., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1904124868

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

TEKNOLOGI HASIL PERIKANAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

Jaka

Versi lain/terkait

Lampiran Berkas

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