CD Skripsi
Aktivitas Ekstrak Kasar Enzim Kolagenasi Dari Organ Dalam Kepala Ular Indonesia (Channa micropeltes) Pada pH Berbeda
Indonesian snakehead (Channa micropeltes) is one type of freshwater fish whose waste has not been utilized properly. The intestines of Indonesian snakehead contain proteolytic enzymes, one of which is collagenase enzyme that degrades polypeptide bonds, especially in fish tissue/collagen in fish. This study aims to determine the maximum pH of collagenase enzyme activity from the internal organs of Indonesian snakehead. This research method uses direct experiments with a non-factorial complete randomized design with the treatment of adding different pH (6, 7, 8) in 3 replicates. The parameters tested in this study were collagenase enzyme activity test based on different pH and measurement of protein concentration in the crude extract of collagenase enzyme. The results showed evidence of an increase in the activity of the crude extract of collagenase enzyme from the internal organs of to Indonesian snakehead caused by the addition of pH as follows: 0.391 U/mL (addition of pH 6), 0.607 U/mL (addition of pH 7), 0.760 U/mL (addition of pH 8). The higher pH addition will caused higher activity of crude extract of collagenase enzyme because it approached the isoelectric point of collagenase enzyme which is in the range of pH 7-8.6. The concentration of protein contained in the crude extract of collagenase enzyme from the internal organs of Indonesian snakehead fish has a value of 186.8 mg/mL. This concentration is relatively high because generally proteases that function to break down proteins are found in the internal organs of fish, especially in parts related to food digestion.
Keywords: Channa micropeltes, extraction, collagenase, internal organs, pH
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