CD Skripsi
Respon Pertumbuhan Eksplan Bonggol Pisang Kepok (Musa Paradisiaca L.) Terhadap Pemberian 6-Benzyl Amino Purine (Bap) Secara In Vitro
Propagation of kepok banana (Musa paradisiaca L.) using seeds from the parent plant is an obstacle for farmers to produce seeds in large quantities and uniformly. in vitro culture is one solution for propagating banana seeds. The growth rate of in vitro plants can be supported by the provision of plant growth regulators (PGR) of the cytokinin type such as 6-Benzyl Amino Purine (BAP). The purpose of this study was to determine the administration of BAP on shoot propagation from kepok banana corm explants in vitro and to determine the effective BAP concentration for shoot propagation. This study used a Completely Randomized Design (CRD) with 7 (seven) treatment levels repeated 5 (five) times to produce 35 experimental units. The treatments consisted of B0: control (without BAP), B1: 0.5 mg/L BAP, B2: 1 mg/L BAP, B3: 1.5 mg/L BAP, B4: 2 mg/L BAP, B5: 2.5 mg/L BAP, B6: 3 mg/L
BAP. The parameters observed were the percentage of live explants. Based on the results of this study, a concentration of 1.5 mg/L BAP gave the highest percentage of live explants, which was 60%. Explants grown from treatments B1, B2, and B5 experienced browning in the middle of the explant growth process. The final condition of the explants looked pale because some explants were contaminated with microorganisms.
Keywords: BAP, in vitro, shoot propagation, Musa paradisiaca L.
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