CD Skripsi
Ekstraksi Dan Pemurnian Astaxanthin Kulit Udang Vannamei (Litopenaeus Vannamei) Menggunakan Pelarut Berbeda
Vannamei shrimp is one of the aquaculture products. By-products of the shrimp processing industry consist of heads and shells. Shrimp shell can be utilized as raw material for astaxanthin which functions as an antioxidant. This study aims to determine the chemical composition, extract yield, functional groups (FT-IR), astaxanthin content, antioxidant activity and qualitative and quantitative purification. The method used was an experimental method with a non-factorial Completely Randomized Design (CRD) of 5 treatment levels, namely hexane, acetone: hexane, acetone, isopropyl alcohol: hexane, and isopropyl alcohol, with a repetition of 3 times. The parameters tested were moisture, ash and fat content, extract yield, functional groups, astaxanthin content, antioxidants and qualitative and quantitative purification. The results showed that the chemical analysis of vannamei shrimp skin flour was 9.51% moisture content, 30.44% ash and 2.06% fat. Extract yields were hexane (0.45%), acetone:hexane (0.57%), acetone (0.73%), isopropyl alcohol:hexane (1.05%) and isopropyl alcohol (1.20%). The functional group profile of the astaxanthin extract showed the presence of astaxanthin compounds with hydroxyl and carboxyl groupmarkers. Theastaxanthin levels were hexane (40.02 μg/g), acetone:hexane (31.29 μg/g), acetone (14.59 μg/g), isopropyl alcohol:hexane (19.34 μg/g), and isopropyl alcohol (17.86 μg/g). The antioxidant activity with the best IC50 was hexane at 450.69 ppm and astaxanthin content after purification was 0.76 ppm.
Keywords: Vannamei shrimp, Different solvents, Astaxanthin, Antioxidant, FT-IR
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