CD Skripsi

Analisis Multilokus Empat Barkode Dna Pada Famili Elaeocarpaceae

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The Elaeocarpaceae family is a group of green flowering plants classified under the Angiosperms, comprising 615 species across 12 genera. This family plays an important role in forest ecosystems, and its wood is utilized in the furniture industry. However, the identification and classification of species within the Elaeocarpaceae family face various challenges due to overlapping morphological characteristics among species. A molecular-based approach, such as DNA barcoding analysis, is therefore a valuable alternative to improve the accuracy of taxonomic identification.This study aims to analyze four DNA barcodes to identify species within the Elaeocarpaceae family. The materials used in this research consist of rbcL, matK, trnL-trnF intergenic spacer, and ITS sequences obtained from the NCBI database. Sequence alignment was carried out using MAFFT v.7, while sequence editing was performed with BioEdit v.7.2. Subsequent DNA sequence analysis and phylogenetic tree construction were conducted using MEGA 11.The results showed that the ITS sequence and the ITS+trnL-trnF IGS combination exhibited the highest proportion of variability. However, in the phylogenetic analysis, the single matK sequence and the multilocus combination of matK+rbcL+trnL-trnF IGS produced more consistent and accurate results, providing clearer genus-level separation within the Elaeocarpaceae family.



Keywoards: Elaeocarpaceae, ITS, matK, rbcL, trnL-trnF intergenic spacer

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2103113260

Penerbit

Pekanbaru : Universitas Riau FMIPA Biologi., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2103113260

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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