CD Skripsi
Eksplorasi Bakteri Endofit Dari Akar Tanaman Hias Hanjuang (Cordyline Fruticosa) Yang Berperan Dalam Bioremediasi Timbal (Pb)
Heavy metal pollution, particularly lead (Pb), is a serious environmental issue that can harm human health and ecosystems. Sustainable biotechnological approaches, such as bioremediation using microorganisms, offer a promising alternative to reduce heavy metal pollution. This study aims to examine the potential of endophytic bacteria from the roots of the Hanjuang plant (C. fruticosa) for lead (Pb) bioremediation. Bacterial isolation was cultured by direct planting method, pure bacterial culture used for partial characterization. The next methods were testing bacteria resistance to heavy metals in nutrient broth medium with 10 ppm Pb. Bacterial growth was measured using spectrophotometer with 600 nm wavelength, and isolate with the highest Optical Density (OD) was selected for biosorption testing in nutrient broth + 10 ppm Pb. Bacterial growth was measured with Total Plate Count (TPC) method and using spectrophotometer at 600 nm wavelength (OD600nm). Lead biosorption was measured using an Atomic Absorption Spectrophotometer (AAS) to determine lead (Pb) biosorption efficiency. The results showed that 17 endophytic bacterial isolats were obtained, each having varied characteristics based on tests. All isolates were resistance to lead (Pb), as indicated by their OD values. Isolate BCF 3.3 had the highest resistance value and having efficiency biosorption percentage of 71%. The findings indicate endophytic bacteria from the roots of C. fruticosa have potential for bioremediation agents against lead pollution in the environment.
Key findings: Atomic Absorption Spectrophotometer, Bioremediation, Cordyline fruticosa, Endophytic Bacteria, Lead (Pb).
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