CD Skripsi

Analisis Multilokus Empat Barkode Dna Pada Famili Arecaceae

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The Arecaceae family, also known as the palm family, is one of the oldest families among flowering plants (Angiosperms). It comprises approximately 200 genera worldwide, with about 25% or around 35 genera and 460 species found in Indonesia. This family is widely utilized for various purposes such as building materials, sources of carbohydrates or food, weaving materials, and ornamental plants. However, the identification and classification of species within the Arecaceae family remain challenging due to the high morphological similarity among species. Therefore, molecular-based approaches, such as DNA barcoding analysis, are required to improve the accuracy of species identification. This study aims to evaluate and analyze four types of DNA barcodes and their combinations to determine the most efficient ones for distinguishing species within the Arecaceae family using a bioinformatics approach. The research steps included sequence retrieval from GenBank, sequence alignment, nucleotide analysis, and phylogenetic tree reconstruction using the Neighbor-Joining method with 1000 bootstrap replicates. The results showed that the ITS region exhibited the highest variation (62.47%), while the rbcL region had the highest level of conservation (92.7%). Based on the phylogenetic tree reconstruction, the single locus matK, the two-locus combinations ITS + trnL-trnF IGS, ITS + matK, and matK + rbcL, as well as the three-locus combinations ITS + trnL-trnF IGS + matK, ITS + trnL-trnF IGS + rbcL, and ITS + matK + rbcL, were effective in clustering genera separately within the Arecaceae family.
Keywords: Arecaceae, DNA Barcoding, Bioinformatics, Phylogenetics,
Multilocus.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2103111471

Penerbit

Pekanbaru : Universitas Riau FMIPA Biologi., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2103111471

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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