CD Skripsi

modifikasi kultur sel punca mesenkimal dengan kombinasi kondisi hipoksia dan paparan lipopolisakarida untuk perlindungan stress oksidatif

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Mesenchymal stem cells (MSCs) are promising candidates for
regenerative therapies, particularly for the treatment of degenerative diseases.
Various strategies have been developed to enhance the therapeutic potential of
MSCs, including culture modification or preconditioning. Hypoxia and
lipopolysaccharide (LPS) exposure individually have been reported to affect cell
viability, proliferation, and antioxidant activity. This study aimed to evaluate the
effects of combined hypoxia and LPS treatment on MSC viability, doubling time,
and the mRNA expression of antioxidant-related genes.
Methods: MSCs were isolated from Wharton’s jelly of human umbilical cords and
cultured under hypoxic conditions using 100 μM cobalt chloride (CoCl₂) and/or
treated with 10 ng/mL LPS. Oxidative stress was induced by the addition of 100 μM
H₂O₂ to the culture medium for 1 h. Cell viability was assessed using the Cell
Counting Kit-8 (CCK-8) assay, and doubling time was calculated using the trypan
blue exclusion method. The mRNA expression levels of SOD1, catalase, and HIF1α
were analyzed using quantitative real-time PCR (qRT-PCR). One-way ANOVA was
used to compare treatment groups.
Results: The combination of hypoxia and LPS for 24 h significantly increased MSC
viability compared with hypoxia alone (p

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2210246768

Penerbit

Pekanbaru : UNRI – PASCA – Ilmu Biomedis., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2210246768

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Magister Ilmu Biomedis

Info Detail Spesifik

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Pernyataan Tanggungjawab

Rahmat

Versi lain/terkait

Lampiran Berkas

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