CD Skripsi
Uji Beberapa Konsentrasi Ekstrak Daun Sirih Hutan (Piper Aduncum L.) Berpelarut Organik Terhadap Mortalitas Ulat Tritipkubis (Plutella Xylostella L.) Di Laboratorium
ABSTRACT
This study aimed to determine the effective concentration of organic solvent-based Piper aduncum L. leaf extract against the mortality of diamondback moth (Plutella xylostella L.) larvae under laboratory conditions. The experiment was conducted using a Completely Randomized Design (CRD) with five concentration treatments (0%, 0.25%, 0.5%, 0.75%, and 1%) and four replications, resulting in 20 experimental units. Each unit was infested with ten third-instar larvae and treated by foliar spraying of the respective extract concentration. Observed parameters included initial mortality time, lethal time 50 (LT₅₀), lethal concentration (LC₅₀ and LC₉₅), daily mortality, total mortality, and changes in larval behavior and morphology. Data were analyzed using analysis of variance followed by the Honestly Significant Difference (HSD) test at a 5% significance level, probit analysis (POLO-PC), and descriptive statistics. The results showed that the 1% concentration achieved a total mortality rate of 82.5%, with an initial mortality time of 4 hours and an LT₅₀ of 30 hours after application. The LC₅₀ and LC₉₅ values were 0.36% and 3.17%, respectively. The highest daily mortality occurred on the first day post-application, followed by a decline on subsequent days. The 1% concentration met the effectiveness criterion for botanical pesticides with organic solvents (≥80% mortality), indicating its potential efficacy in controlling P. xylostella. In conclusion, organic solvent-based P. aduncum leaf extract demonstrates strong potential as an environmentally friendly botanical insecticide, with an optimal concentration of 1% for effective management of diamondback moth larvae in cabbage crops under laboratory conditions.
Keywords: cabbage, Plutella xylostella L., Piper aduncum L., botanical insecticides
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