CD Skripsi
isolasi dan identifikasi bakteri pada ikan mas (cYPRINUS CARPIO) keramba jaring apung di waduk koto panjang sebagai rancangan e-booklet kelas x
Goldfish (Cyprinus carpio) is a freshwater fishery commodity with high economic value and is widely cultivated in floating net cages (KJA) in the Koto Panjang Reservoir. However, intensified cultivation has led to an increase in organic waste and triggered pathogenic bacterial infections, often leading to mass mortality of carp. Bacteria can be found on the surface and internal organs of fish, and some are pathogenic and pose a health risk to humans if consumed. Therefore, identifying bacteria in carp is crucial to support sustainable cultivation and serves as an indicator of human health if consumed.
Data on bacteria in carp (Cyprinus carpio) is crucial and offers significant benefits, including as an alternative learning medium in schools and universities. This data can be used as a learning reference, including in the classification of living things, which is used theoretically both in class and outside the classroom, through e-booklets. The e-booklet was chosen as a supplementary reference and to facilitate students' understanding of the classification of living things. This e-booklet discusses the classification and characteristics of bacteria in carp (Cyprinus carpio) in floating net cages (KJA) in the Koto Panjang Reservoir.
This is a qualitative descriptive study using experimental methods to determine the types of bacteria in carp (Cyprinus carpio). Carp samples were collected in floating net cages in the Koto Panjang Reservoir. The isolation and identification of bacteria in carp (Cyprinus carpio) was conducted at the Laboratory of the Faculty of Medicine, University of Riau. This study was conducted from December 2024 to March 2025. Identification included macroscopic and microscopic identification, as well as biochemical tests.
The results of the study of bacterial isolates in carp (Cyprinus carpio) showed four types of isolates with distinct macroscopic and microscopic morphological characteristics. Macroscopically, the isolates showed round or irregular colony
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shapes, with color variations such as yellowish white, bluish green, and grayish white, as well as flat or convex colony elevations and flat or wavy edges. Microscopically, all isolates were bacilli (rods) and classified as Gram-negative bacteria that appeared pink after Gram staining. The TSIA biochemical test showed that the isolates were able to ferment certain sugars, indicated by changes in media color, with some isolates producing H₂S or not. In the SCA test, some isolates showed the ability to use citrate as a carbon source, indicated by a change in media color from green to blue. The SIM test showed that all four isolates were motile, as evidenced by spreading growth on the media. Further identification using Bergey's Manual showed that isolates IIIWa1 and IUIWa2 resembled Proteus sp., IIIWa2 resembled Pseudomonas sp., and IUIWa1 resembled Escherichia coli. Based on the validity analysis of the results of the research on the identification of bacteria in goldfish (Cyprinu carpio) in Koto Panjang Reservoir floating net cages, the E-Booklet design for the Classification of Living Things material for Grade X of Senior High School is very valid and can be used as a teaching medium in the Classification of Living Things material to increase student motivation and is designed in the form of an E-Booklet.
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