CD Skripsi
Potensi Ekstrak Teripang Berunok (Paracaudina Australis) Sebagai Anti-Kolesterol
This study aimed to identify the secondary metabolites contained in the extract of berunok sea cucumber (Paracaudina australis) and to evaluate its in-vitro anti-cholesterol activity. Berunok sea cucumber is one of Indonesia‟s marine biota with potential as a natural source of bioactive compounds that can help prevent degenerative diseases. The extraction was carried out using the maceration method with methanol solvent for 48 hours (T1), 72 hours (T2), and 96 hours (T3). Phytochemical screening was conducted to detect the presence of secondary metabolites such as flavonoids, alkaloids, saponins, phenols, and terpenoids/steroids. Anti-cholesterol activity was analyzed using a UV-Vis spectrophotometer based on cholesterol inhibition percentage. The results showed that the berunok sea cucumber extract contained saponins, and terpenoids, while flavonoids and phenols were not detected. The anti-cholesterol test indicated that maceration time had a significant effect on cholesterol inhibition activity. The highest activity was found at 48 hours of maceration (35.54 mg/mL), decreasing at 72 hours (44.08 mg/mL), and reaching the lowest at 96 hours (49.86 mg/mL). These findings suggest that maceration duration plays a crucial role in determining the extraction efficiency of bioactive compounds. Therefore, berunok sea cucumber extract (Paracaudina australis) has promising potential as a natural source of anti-cholesterol compounds and could be further developed for pharmaceutical and nutraceutical applications.
Keywords: anti-cholesterol,berunok,maceration,paracaudinaaustralis,time
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