CD Skripsi
Pengaruh Ph Berbeda Terhadap Aktivitas Ekstrak Kasar Enzim Kolagenase Dari Jeroan Ikan Gabus (Channa Striata)
Snakehead fish is a freshwater fish that can be found throughout Indonesian
waters. Snakehead fish contains 13.44% albumin, protein content of 15.99%
which is dominated by the amino acid leucine (0.54%) and the amino acid glutamate (1.45%). It also contains minerals dominated by iron (6.22%) and zinc (2.24%), both of which play a role in the immune system and wound healing, but leaving the innards that are still little utilized. One method developed in fishery waste processing is converting waste into other products that have added value or sales value such as hydrolase enzymes. This study aims to increase the utilization of snakehead fish waste to increase added value, namely as a source of collagenase enzymes. The method used in this study is an experiment, namely extracting collagenase enzymes from snakehead fish innards.Each type of treatment was repeated three times, resulting in a total of nine experimental units. The research design used was a non-factorial Completely Randomized Design (CRD). Observation parameters in this study included proportion tests, proximate tests, collagenase enzyme activity tests, and dissolved protein concentrations. The conclusion from the results of this study is that differences in pH concentration in enzyme activity testing affect the resulting values. At pH 7 (1,165 U/mL), pH 8 (1,456 U/mL) and pH 9 (0.853 U/mL). Determination of the activity of the crude extract of collagenase enzyme extracted from snakehead fish innards was carried out to obtain useful data in the application of collagenase. The specific activity of the crude extract of collagenase enzyme from snakehead fish innards obtained a value (0,792 U/mg)
Keywords: Snakehead fish, Offal, Hydrolase Enzyme
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