CD Tesis

keanekaragaman aksesi sagu (Metroxylon sagu) berdasarkan penanda morfologi, komponen produksi dan molekuler (metode start codon targeted)

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Sago (Metroxylon sagu) is a plant that has high starch potential, but the development of information in terms of DNA markers is still limited. This study aims to analyze and examine the diversity of sago accessions based on morphological markers, yield components, and molecular using the SCoT method. This study used a Split Plot Design, the main plot consisted of 3 locations (blocks D35, A38, and C33) and the subplots consisted of 2 accessions (Molat and Tuni), each location was observed as many as 30 clumps so that the total sample plants were 90 clumps. The variables observed were 9 variables for morphological characters, 9 variables for yield component characters, and 10 variables for molecular characters.
The results showed that the main plot factor had a significant effect on the morphological characters and yield components of sago. Based on the analysis of variance, Molat accessions showed better results than Tuni, especially accessions in blocks location A38 and D35, the interaction between location and accessions with the combination of A38 x Molat gave optimum results. Similar results were shown from the scoring analysis which showed that the 5 best rankings came from block A38 with Molat accessions (AM8, AM3, AM7, AM15, and AM10). The correlation analysis showed that the variables related to plant height, stem diameter, number of log, log diameter, and log weight had a close relationship with the yield variable, namely starch potential per stem, in line with the results of the path analysis which showed that the variables plant height (11.57), leaf area (6.56), rachis length (5.04), number of frond (4.38), and log weight (2.14) had a direct positive effect on starch potential per stem.
The results of molecular analysis showed that the SCoT marker was effective and efficient in evaluating the diversity of sago accessions as seen from the value of 4.71 alleles per locus, PIC 44%, the smallest PI of 0.93, shared alleles of 72.73%, specific alleles 18.18%, Fst (0.14), Gst (0.12), A of 2.67, Ae value of 1.92, Ho ranging from 0.56–0.67, He value ranging from 0.38 to 0.48, and Nm value of 4.37. Based on these results, it can be concluded that primers 13, 30, and 33 can be used to identify the diversity of sago accessions observed. The results of molecular analysis differ from the results of variance analysis and scoring analysis which show that Molat and Tuni accessions in block location C33 have specific alleles, different from accessions in other blocks.
Keywords: Accession, genetic diversity, molat, sago, and tuni

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2310246665

Penerbit

Pekanbaru : UNRI – PASCA – Magister Ilmu Pertanian., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2310246665

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Magister Ilmu Pertanian

Info Detail Spesifik

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Pernyataan Tanggungjawab

Rahmat

Versi lain/terkait

Lampiran Berkas

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