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Perbandingan Ekspresi Gen Hepatocyte Growth Factor Dan Vascular Endothelial Growth Factor Pada Sel Punca Mesenkimal Dengan Suplementasi Human Platelet Lysate Dan Fetal Bovine Serum

Mesenchymal stem cells (MSCs) are multipotent stem cells with the ability to proliferate, differentiate, and immunomodulate. Mesenchymal stem cells produce biological coumpounds such as growth factors, cytokines, and chemokines, which have high therapeutic potential for various diseases. This study was motivated due to the high zoonotic potential and ethical concerns associated with the use of fetal bovine serum (FBS) as a supllement in MSC culture, so we use human platelet lysate (HPL) as alternative serum. The aim of this study was to compare the effects of HPL and FBS supplementation in culture media on the expression of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) in MSCs isolated from the umbilical cord. This study used a quasi-experimental in vitro design. Mesenchymal stem cells were isolated from the umbilical cord and cultured using the explant method with 5% HPL and 10% FBS (control) supplementation. The expression levels of HGF and VEGF were evaluated using relative reverse transcription polymerase chain reaction (RT-PCR) method and analyzed with GraphPad. An independent T-test was used to assess the differences in HGF and VEGF expression between the HPL and FBS supplemented groups. The results showed no significant differences in HGF (p = 0,9) or VEGF (p = 0,65) expression between 5% HPL and 10% FBS groups. The conclusion of this study is that HPL supplementatin in MSC cultures does not affect HGF or VEGF expression, suggesting that HPL can serve as an alternative to FBS for MSC culture supplementation.
Keywords: Mesenchymal stem cells, human platelet lysate, fetal bovine serum, hepatocyte growth factor, vascular endothelial growth factor, RT-PCR.

Ketersediaan
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Perpustakaan Universitas Riau 2208112459
2208112459
Tersedia
Informasi Detail
Judul Seri
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No. Panggil
2208112459
Penerbit
Pekanbaru : Universitas Riau – Fakultas Kedokteran - Pendidikan Kedokteran.,
Deskripsi Fisik
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Bahasa
Indonesia
ISBN/ISSN
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Klasifikasi
2208112459
Tipe Isi
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Tipe Media
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Tipe Pembawa
-
Edisi
-
Subjek
PENDIDIKAN KEDOKTERAN
Info Detail Spesifik
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Pernyataan Tanggungjawab
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Tidak tersedia versi lain

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