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Perbandingan Ekspresi Gen Interleukin 6 Dan Tumor Necrosis Factor Alpha Pada Sel Punca Mesenkimal Yang Disuplementasi Fetal Bovine Serum Dan Human Platelet Lysate

Mesenchymal stem cells (MSCs) possess immunomodulatory properties that are essential for the development of regenerative therapies. In MSC culture, serum selection is critical because fetal bovine serum (FBS) carries a risk of xenogeneic immunization, making human platelet lysate (HPL) a more biocompatible alternative. This study aimed to analyze the differences in the expression of the Interleukin-6 (IL-6) and Tumor Necrosis Factor-Alpha (TNF-α) genes in MSCs supplemented with 5% HPL compared to 10% FBS. This in vitro experimental study with a post-test control group design was conducted from January to December 2025 at a private hospital in Pekanbaru and the Integrated Biomedical Laboratory LONTAR, Faculty of Medicine, Universitas Riau. MSCs derived from Wharton’s jelly were isolated using the explant method and cultured in two treatment groups, namely 10% FBS (n=5) and 5% HPL (n=6). Once the cultures reached stable conditions, the relative mRNA expression levels of IL-6 and TNF-α were analyzed using RT-qPCR, with GAPDH serving as the housekeeping gene for normalization via the ΔΔCt method. Statistical analysis was performed using the Independent Samples T-Test. The results showed no significant differences in the relative mRNA expression of IL-6 (p = 0.91) or TNF-α (p = 0.30) between the HPL and FBS groups. These findings indicate that 5% HPL does not alter the paracrine expression profile of MSCs, particularly IL-6 and TNF-α, and may serve as a safe xenogeneic-free serum alternative for MSC culture supplementation.

Keywords: Fetal Bovine Serum, Human Platelet Lysate, Interleukin 6, Mesenchymal Stem Cells, Tumor Necrosis Factor Alpha

Ketersediaan
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Perpustakaan Universitas Riau 2208112461
2208112461
Tersedia
Informasi Detail
Judul Seri
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No. Panggil
2208112461
Penerbit
Pekanbaru : Universitas Riau – Fakultas Kedokteran - Pendidikan Kedokteran.,
Deskripsi Fisik
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Bahasa
Indonesia
ISBN/ISSN
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Klasifikasi
2208112461
Tipe Isi
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Tipe Media
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Tipe Pembawa
-
Edisi
-
Subjek
PENDIDIKAN KEDOKTERAN
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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Tidak tersedia versi lain

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