CD Skripsi

Perbandingan Ekspresi Gen Interleukin-8 Dan Transforming Growth Factor-Β Pada Sel Punca Mesenkimal Dengan Suplementasi Human Platelet Lysate Dan Fetal Bovine Serum

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Mesenchymal stem cells (MSCs) have great potential in regenerative therapy, but conventional culture using fetal bovine serum (FBS) poses biological and ethical risks, making human platelet lysate (HPL) a safer alternative. This study aims to compare the expression of interleukin-8 (IL-8) and transforming growth factor-β (TGF-β) genes in MSCs cultured using HPL and FBS supplementation. This experimental study uses a post-test only control group design. Mesenchymal stem cells were isolated from Wharton's Jelly of the umbilical cord and cultured to the third passage before being divided into two groups: 5% HPL culture and 10% FBS culture (n=6 each). IL-8 and TGF-β mRNA expression was measured using RT- qPCR and analyzed using the ΔΔCt method, followed by statistical analysis using the Shapiro–Wilk test and independent t-test. The study results showed that IL-8 and TGF-β expression in the FBS group was slightly higher than in the HPL group (IL- 8: 1.024 vs. 0.920; TGF-β: 1.106 vs. 0.946), but there was no significant difference (p>0.05).


Keywords : mesenchymal stem cells, human platelet lysate, fetal bovine serum, IL- 8, TGF-β, Wharton’s Jelly.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2208112098

Penerbit

Pekanbaru : Universitas Riau – Fakultas Kedokteran - Pendidikan Kedokteran., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2208112098

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PENDIDIKAN KEDOKTERAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

Vina

Versi lain/terkait

Lampiran Berkas

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