CD Skripsi
Aktivitas Dekonjugasi Dan Antimikrob Oleh Limosilactobacillus Fermentum Serta Pediococcus Pentosaceus Dengan Atau Tanpa Penggunaan Enkapsulan Dari Daun Kelapa Sawit
ABSTRACT
Probiotics are living microorganisms that have the ability to deconjugate
bile salts and inhibit pathogenic bacteria. The activity of bile salt deconjugation and
the inhibition of pathogenic bacteria need to be carried out on Limosilactobacillus
fermentum InaCC B1295 and Pediococcus pentosaceus strain 2397, with and
without cellulose microfiber (CMF) hydrogel encapsulation from oil palm leaves.
This study was conducted experimentally using a completely randomised design
with four treatments: Limosilactobacillus fermentum InaCC B1295 and P.
pentosaceus strain 2397 cells encapsulated with or without CMF hydrogel from oil
palm leaves. Each treatment was repeated four times to obtain 16 experimental
units. The parameters observed in this study were the bile salt deconjugation test
and the inhibition of Gram-positive and Gram-negative pathogenic bacteria. The
results showed that L. fermentum InaCC B1295 and P. pentosaceus strain 2397
encapsulated with or without CMF hydrogel from oil palm leaves significantly
affected the deconjugation activity of sodium taurocholate and antimicrobial
activity against S. aureus, L. monocytogenes, E. coli, but did not significantly
influence the deconjugation activity of glycocholic acid and antimicrobial against
Salmonella sp. The diameter of the precipitation zone in glycocholic acid ranging
from 6,68–7,75 mm was larger than that of sodium taurocholate ranging from 4,43–
6,33 mm. In general, L. fermentum InaCC B1295 and P. pentosaceus strain 2397,
encapsulated with or without CMF hydrogel, show high antimicrobial activity
against S. aureus, L. monocytogenes, and E. coli. In contrast, both LAB from oil
palm leaves, encapsulated with or without CMF hydrogel, showed relatively low
antimicrobial activity against Salmonella sp.
Keywords: Probiotics, Limosilactobacillus fermentum, Pediococcus pentosaceus,
cellulose microfiber, oil palm leaves
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