CD Skripsi
Profil Fekal Mikroflora Pada Orang Dewasa Yang Diberi Susu Ultra High Temperature Dengan Atau Tanpa Limosilactobacillus Fermentum Inacc B1295 Yang Dienkapsulasi Dengan Hidrogel Cellulose Microfiber Dari Batang Kelapa Sawit
ABSTRACT
Probiotics are microorganisms that provide health benefits when
consumed in adequate amounts by contributing to the balance of intestinal
microflora. Limosilactobacillus fermentum InaCC B1295, a local strain isolated
from fermented buffalo milk (dadih), is known for its resilience in the
gastrointestinal tract and its potential to support digestive function. This study
aimed to evaluate the fecal microflora profile of adult males consuming ultrahigh-
temperature milk with or without encapsulated L. fermentum InaCC B1295
using cellulose microfiber hydrogel derived from oil palm trunk. A 60-day
intervention was conducted on two independent groups. Observed parameters
included total plate count, lactic acid bacteria, Bifidobacterium spp., and
pathogenic bacteria such as E. coli, Salmonella spp., Shigella spp., and Klebsiella
spp. Statistical analysis was performed using an independent sample t-test with
IBM SPSS version 25 at a 5% significance level. The total plate count reached
12.12 CFU/g in the non-probiotic group and 12.38 CFU/g in the probiotic group.
Lactic acid bacteria were recorded at 8.86 CFU/g and 9.16 CFU/g, while
Bifidobacterium spp. showed values of 7.22 CFU/g and 7.20 CFU/g. E. coli
counts were 8.68 CFU/g and 9.11 CFU/g. Salmonella spp. and Shigella spp. were
lower in the probiotic group at 2.83 CFU/g compared to 4.22 CFU/g. A significant
reduction was found in Klebsiella spp., with 6.44 CFU/g in the probiotic group
and 5.57 CFU/g in the placebo group. Although significant differences were only
observed for Klebsiella spp., metagenomic analysis revealed genus-level
variations in the digestive microflora following probiotic administration.
Keywords: Limosilactobacillus fermentum, cellulose microfiber, UHT milk,
probiotics, fecal microflora
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