CD Skripsi
Uji Antagonis Bacillus Spp. Dalam Menghambat Jamur Marasmius Palmivorus Sharples Penyebab Penyakit Busuk Tandan Kelapa Sawit Secara In Vitro
ABSTRACT
Oil palm (Elaeis guineensis Jacq.) is an important plantation crop in Indonesia due to its high economic value as a source of palm oil and palm kernel oil used in various food and non-food industries. Productivity is often disrupted by bunch rot disease caused by the fungus Marasmius palmivorus Sharples, which can lead to yield losses of up to 25%. Chemical pesticides are commonly applied to control this disease, but their use poses environmental and health risks. Therefore, biological control using Bacillus spp. offers a more environmentally friendly alternative. This study aimed to evaluate the antagonistic activity of different Bacillus spp. against M. palmivorus and determine their effects on the morphological characteristics of the pathogen in vitro. The research consisted of descriptive observation and experimental tests including inhibition assay, inhibition zone assay, and filtrate inhibition assay using a completely randomized design (CRD) with 5 treatments and 4 replications. The results showed that B. amyloliquefaciens, B. cereus, and B. velezensis were able to inhibit the growth of M. palmivorus, while only B. velezensis produced a clear inhibition zone with an inhibition rate of 66.11% and zone formation of 14.82%. Filtrate inhibition was highest in B. amyloliquefaciens at 53.75%. Morphological observation revealed that Bacillus spp. caused abnormalities in fungal hyphae, including swelling, plasmolysis, coiling and lysis. These findings indicate that Bacillus spp., particularly B. velezensis, have potential as biological control agents against bunch rot disease in oil palm.
Keywords: Bacillus spp., Marasmius palmivorus, oil palm, bunch rot disease
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