CD Skripsi
Pengaruh Kombinasi Ga3 Dengan Bap Dan Naa Terhadap Teknik Kultur In Vitro Embrio Kelapa Sawit (Elaeis Guineensis Jacq.)
Oil palm (Elaeis guineensis Jacq.) is an important commodity in Indonesia due to its high economic value as a source of vegetable oil and its contribution to national foreign exchange. The continuous expansion of plantation areas increases the demand for planting materials; however, generative propagation requires a long germination period due to seed dormancy. Tissue culture offers an alternative to rapidly produce planting materials. This technique involves the in vitro cultivation of plant cells, tissues, or organs under aseptic and controlled conditions using artificial media supplemented with plant growth regulators (PGRs). This study aimed to evaluate the effects and optimal concentrations of gibberellic acid (GA₃), as well as its combinations with naphthalene acetic acid (NAA) and benzylaminopurine (BAP), on the in vitro culture of oil palm embryos. A completely randomized design was used with ten treatments and three replications. Observed parameters included time to plumule emergence, time to radicle emergence, plumule length, root length, number of leaves, and number of roots. Data were analyzed using analysis of variance followed by Duncan’s New Multiple Range Test at a 5% significance level. The results showed that GA₃, NAA, and BAP significantly affected the time of plumule emergence but had no significant effect on other growth parameters. The combination of 1 mg/l GA₃ + 1 mg/l BAP resulted in the fastest plumule emergence, while 5 mg/l GA₃ + 1 mg/l BAP produced the longest plumule.
Keywords: Oil palm, tissue culture, gibberellic acid, plant growth regulators
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