CD Skripsi
Pengaruh Kombinasi Konsentrasi Naa Dan Bap Pada Media Ms Terhadap Kultur In Vitro Embrio Kelapa Sawit (Elaeis Guineensis Jacq.)
ABSTRACT
Oil palm (Elaeis guineensis Jacq.) is a strategic agricultural commodity and
a major source of vegetable oil with high productivity, making the availability of
high-quality planting material a critical factor for sustainable plantation
development. In monocotyledonous species such as oil palm, effective vegetative
propagation can only be achieved through tissue culture techniques, including
embryo culture. This study aimed to evaluate the effects of different combinations
of the plant growth regulators α-naphthaleneacetic acid (NAA) and 6-
benzylaminopurine (BAP) on the in vitro growth of oil palm embryo explants of the
Dura variety cultured on Murashige and Skoog (MS) medium. The experiment was
arranged in a completely randomized design with a single factor consisting of ten
NAA and BAP combinations and three replications. Observed parameters included
the time to plumule and radicle emergence, plumule length, root length, number of
leaves, and explant viability. Data were subjected to analysis of variance followed
by Duncan’s New Multiple Range Test at the 5% significance level. The results
demonstrated that the combinations of NAA and BAP significantly affected all
observed growth parameters of oil palm embryos cultured in vitro. The combination
of 0.1 mg/l NAA and 1.0 mg/l BAP was most effective in initiating plumule
development, whereas 1.0 mg/l NAA combined with 0.1 mg/l BAP predominantly
promoted root initiation. These findings confirm that the balance between auxin and
cytokinin plays a crucial role in directing organ differentiation during in vitro
embryo development of oil palm.
Keywords : oil palm, embryo culture, NAA, BAP, in vitro
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