CD Skripsi
Deteksi Polutan Palm Oil Mill Effluent Berdasarkan Respirasi Dissolved-Oxygen Dan Co2 Berbasis Biosensor Mikroalga Dan Sensor Ndir
The increasing production of palm oil in Indonesia each year has led to a rise in palm oil waste generation, including Palm Oil Mill Effluent (POME), which is produced at approximately 600–700 liters per ton of Fresh Fruit Bunches (FFB). This effluent contributes to pollution in aquatic ecosystems due to its dark coloration and high Chemical Oxygen Demand (COD) levels. Therefore, rapid and real-time monitoring of POME pollutants is essential. This study aims to detect POME pollutants based on the dissolved oxygen (DO) and carbon dioxide (CO2) respiration rates of Nannochloropsis oculata using an amperometric biosensor and an NDIR sensor. The experiments were conducted by adding POME at varying concentrations of 5%, 10%, 15%, 20%, and 25%, while CO2 gas was supplied at a flow rate of 5–10 L/min for 60 seconds into the microalgae cultures. The results indicated an increase in DO concentration and CO2 absorption with higher POME concentrations, attributed to the enhanced photosynthetic activity of Nannochloropsis oculata. This was validated by the observed increase in cell density, determined through optical density (OD) measurements. Chlorophyll analysis revealed that the highest chlorophyll-a content occurred at the 10% POME concentration, indicating that the optimal POME level positively influenced microalgal health. Based on the measurements, the sensitivity of the electrode sensor in detecting DO variations and that of the NDIR sensor in detecting CO2 absorption changes were 2.57 %DO/(%POME) and 0.3 ppm/(%POME), respectively. These findings demonstrate that Nannochloropsis oculata functions effectively as a bioreceptor in sensors for detecting POME pollutants while simultaneously contributing to pollutant reduction by utilizing the nutrients present in POME.
Keywords: Amperometric biosensor, Chlorophyll-a, CO2 absorption, Dissolved oxygen, NDIR sensor, Nannochloropsis oculata,
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