CD Skripsi
Isolasi Dan Uji Antagonis Isolat Jamur Asal Rizosfer Bawang Merah (Allium Ascalonicum L.) Terhadap Alternaria Porri Ellis Cif. Secara In Vitro Serta Identifikasi Jamur Yang Berdaya Antagonis Tinggi
The research aims to isolate and to obtain isolates which have a high antagonicity against A. porri in vitro. This research has been conducted at Plant Pathology and Plant Pest Laboratory, Agriculture Faculty, University of Riau from May until July 2017. The research consisted of 6 steps as follow: 1) isolation and purification of the rhizosphere fungus from onion plant used exploration method, 2) antagonicitytest of rhizosphere fungus from onion plant againstA. porri used experiment method, 3) hyperparasitism test of 5 rhizosphere fungus which have a high antagonicityused observation method, 4) growth test of the 5 rhizosphere fungus from onion plant which have a high antagonicityused experiment method, 5) hypovirulency test of 5 rhizosphere fungus which have a high antagonicityused observation method and 6) identification of the 5 rhizosphere fungus from onion plant which have a high antagonicityused observation method. Data collected from step 1, 3, 5 and 6 were descriptively analyzed. Data from step 2 and 4 were analyzed by using analysis of variance. The mean of each treatment were compared with Duncan’s New Multiple Range Test(DNMRT) at level 5%. Result of the research showed that there were 16 isolates, 5 isolates had a high antagonicity: isolate R6, isolate R11, isolate R10, isolate R4 dan isolate R5. The isolate R6 had the best inhibition growth to A. porri (59,90%), the biggest diameter of the growth (90,00 mm) and the most rapid colony growth (25,00 mm/day). The isolate R6, isolate R11, isolate R10 and isolate R5 were hypovirulent and isolat R4 were virulent. The isolatesbelonged to genera: Trichoderma (isolate R6), Aspergillus (isolate R11), Rhizoctonia (isolate R10), Fusarium (isolate R4) and Humicola (isolate R5).
Keyword: Isolation, antagonicity, identification, rhizosphere fungus, onion plant
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