CD Skripsi

Fraksinasi Metabolit Sekunder Dan Uji Antimikroba Media Fermentasi Batch Penicillium sp. LBKURCC34

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ABSTRACT

Penicillium sp. LBKURCC34 is a fungi isolated from peat soil of primary forest at Giam Siak Kecil Bukit Batu (GSKBB), Biosphere Reserve in Riau Province. This fungus has ability to produce hydrolytic enzyme chitinase. The purpose of this study is to separate crude extract of secondary metabolites from the growth media of Penicillium sp. LBKURCC34 that have an antimicrobial activity. Secondary metabolite production was done by fermentation in a liquid medium inoculated with spores of Penicillium sp. LBKURCC34 (7x1012 spores in 50 mL of media). The fermentation was carried out for 14 days in the media and extracted with ethyl acetate. The ethyl acetate extract was evaporated, separated by Column Chromatoghraphy (CC) and the concentrate fraction from CC was dissolved by methanol. Phytochemicals test was applied to the extract. Antimicrobial tests were performed by the disc diffusion method toward Escherichia coli, Staphylococcus aureus, Staphylococcus ephidermidis, Bacillus subtilis, and, Candida albicans growth using 3 concentration of crude extracts (1,9; 3,8; and 5,7 mg/mL). Amoxicilin® and Ketokonazol® were used as the positive control. The result of phytochemical test showed positif results for terpenoid and fenolic. Antimicrobial activity of all pathogenic microbes showed that the increase in the concentration of crude ethyl acetate extract did not increase antimicrobial activity and was generally lower than positive control. An identification was performed on Thin Layer Chromatography (TLC) with eluent ethyl acetate : hexane (6:4) giving 7 spots under UV (254 mm) and 8 spots under UV(366) nm . Results of TLC after spraying with 0.5% p-anisaldehyde showed the presence of red peptaibol compounds. The results were confirmed by High Performance Liquid Chromatography (HPLC) that showed 3 peaks under UV detector (210 and 365 nm). The fractions of the KK produce 11 fractions. Antimicrobial activity in Escherichia coli and Candida albicans in 1-5 fractions showed no activity.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 02.119 (0008)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2019

Deskripsi Fisik

xiii, 39 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 02.119 (0008)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

JAKA

Versi lain/terkait

Lampiran Berkas

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