CD Skripsi
Produksi Enzim Selulase Dan Xilanase Dari Eupenicillium Javanicum Dengan Substrat Kulit Nanas Menggunakan Solid-State Fermentation
The utilization of enzymes in bioprocess industry increases annually. Enzyme ability to accelerate reaction and its biodegradability make it preferable to be utilized as catalyst. Cellulase and xylanase are ones of common enzymes that used in industries, such as pulp and paper, detergent, syrup and more. Pineapple peel waste has potential lignocellulose content to substitute pure substrate such as cellulose and xylan which is not efficient in cost. Solid-state fermentation allows microorganism to live in condition that suits its habitat. This research aims to pH and temperature of fermentation’s effect on cellulase and xylanase enzyme production both in solid-state and submerged fermentation by comparing the enzyme activity resulted. Fermentation for enzyme production was done for 96 h at temperature (25, 30, 35 oC) and pH (4, 6, 8). Enzyme activity was obtained with DNS method using spectrophotometry visible at 540 nm. The optimal condition to produce highest enzyme activity was obtained at temperature 30 oC and pH 6, cellulase and xylanase reach 0,155 U/mL and 1,006 U/mL.
Keywords : cellulase, enzyme, eupencillium javanicum, solid-state fermentation, submerged fermentation, xylanase
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