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Isolasi Dan Karakterisasi Ralstonia Solanacearum Penyebab Penyakit Layu Bakteri Pada Bibit Eucalyptus Pellita Klon 077 Aa Dan Penghambatannya Oleh Pseudomonad Fluorescens
The research aims to isolate and characterize Ralstonia solanacearum cause bacterial wilt on seedlings of Eucalyptus pellita clon 077 AA and its inhibition by Pseudomonad fluorescens. The research was conducted at Laboratory of Plant Protection Department PT. Arara Abadi Group Sinarmas, Subregency of Tualang, Regency of Siak, Province of Riau from July to September 2019. The research was conducted using exploration and observation method and then analyzed descriptively. The observation parameters were morphological characteristic, physiological characteristic, virulency, inhibition ability by Pseudomonad fluorescens and bacterial race. Isolation of phatogenic bacteria from Eucalyptus pellita clon 077 AA obtained two isolate which has similar morphological characteristic one each other. Physiological characteristic shows that isolate Rs-2 has similar character to Ralstonia solanacearum on colony colour in medium NAPSA+TZC, negative reaction on gram test, negative reaction on gram staining test, positive reaction on oxidase test and positive reaction on OF test. Virulency test of isolate Rs-2 on seedlings of Eucalyptus pellita clon 077 AA shows positive reaction so isolate Rs-2 is pathogenic. The inhibition zone that produced by Pseudomonad fluorescens towards Ralstonia solanacearum isolate Rs-2 is 17,3 mm in medium King’s B and 12,4 mm in medium NAPSA and classifies as strong and the mechanism of its inhibition is bacteriostatic. The pathology race test shows that Ralstonia solanacearum isolate Rs-2 is Ralstonia solanacearum on race 1.
Keywords : characterization, Eucalyptus pellita seedlings clon 077 AA, isolation, Pseudomonad fluorescens, Ralstonia solanacearum
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