CD Skripsi
Isolasi dan Seleksi Fungi Endofit dari Akar Mangrove Muara Sungai Siput Sebagai Sumber Senyawa Antibakteri
Mangrove plant is one of the medical plants that has been being used to treat some illnesses. Mangrove strives under extreme conditions such as highly fluctuating salinities and humidities in anaerobic soils. Therefore, mangroves are potential sources for the discovery of new compounds and microbiota with extensive applications in pharmaceutical science. Endophytic fungi are one of the associated host plant microbiota through mutualism symbiotic. Endophytic fungi have been known as sources of novel bioactive compounds. This study aims to isolate endophytic fungi from the roots of mangrove plants and to screen some isolates with the ability to inhibit the growth of pathogenic bacteria. A total of 15 endophytic fungi have been isolated from the roots of mangrove that growth at Sungai Siput, Bengkalis Regency. These 8 mangroves samples have been identified as seven different species i.e., Aegiceras sp., Lumnitzera racemosa Wild., Avicennia marina (Forssk.) Vierh., Laguncularia racemosa (L) C. F. Gaertn., Sonneratia ovata Backer., Kandelia candel (L) Druce., and Xylocarpus granatum J. Koenig. The ability of endophytic fungi to inhibit the growth of pathogenic bacteria were determined by conducting antagonistic study against Escherichia coli, Vibrio parahaemolyticus, Vibrio algynolyticus, Bacillus subtilis, and Staphylococcus aureus. Overall, using Tukey assay with 95% confidence level, our finding showed that the highest antibacterial activity were produced by the isolates of 2, 5, and 15, which were isolated from the roots of mangrove Lumnitzera racemosa Wild., Avicennia marina (Forssk.) Vierh., dan Xylocarpus granatum J. Koenig., respectively. These isolates were able to inhibit the growth of both Gram-positive and negative bacteria. Three highest inhibition zones were produced by the isolates of 2 against Vibrio parahaemolyticus (9,85±0,52 mm) and Bacillus subtillis (18,20±0,98 mm), and isolate 5 against Staphylococcus aureus (14,83±0,47 mm). This result showed the potency of mangrove endophytic fungi as alternative sources of antibacterial compounds.
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