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Penentuan Kemurnian Madu Menggunakan Spektroskopi Fluoresensi Dan Spektroskopi Fourier Transform Infrared (Ftir)
Fluorescence Spectroscopy and Fourier Transform Infrared (FTIR) spectroscopy systems have been used as nondestructive methods that have the potential to test honey purity. Fluorescence spectroscopy system consists of 405 nm diode laser, mirror, ND filter and USB 2000+ Ocean Optics spectrometer, while FTIR spectroscopy system consists of FTIR Prestige 21 / FTIR 8400 spectrometer. Fluorescence spectroscopy was used to determine the relation between fluorescence intensity to the dominant fluorescence wavelengths. FTIR spectroscopy was used to analyze the spectra produced in accordance with peaks formed from the results of sample measurements. Samples consisted of 6 forest honey and 4 branded honey (BPOM certified, MUI and DEPKES permit), 1 date syrup, 1 corn syrup and 4 honeys that mixed with corn syrup. Honey and non-honey samples were recorded using a USB 2000+ Ocean Optics spectrometer. The values of sugar contents were measured manually using a refractometer brix. The values of the dominant fluorescence wavelengths and the values of sugar contents were analyzed. The result showed that the highest fluorescence intensity is 1016.92 a.u at wavelength of 515.08 nm, while the lowest fluorescence intensity is 185.31 a.u at wavelength of 641.30 nm that indicated by a non honey sample. The results showed that the spectrum of pure honey samples peaked at an area with wavelength of 500 nm. The difference of FTIR spectrum shape is shown by the honey samples which is not given an adulteration, where the difference begins to be seen clearly at the wave number range 1150 cm-1 to 650 cm-1.
Keywords: fluorescence spectroscopy, FTIR spectroscopy, fluorescence wavelength, wave number, honey
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