CD Skripsi
Penentuan Kemurnian Madu Menggunakan Spektroskopi Fluoresensi Dan Spektroskopi Fourier Transform Infrared (Ftir
ABSTRACT
Fluorescence Spectroscopy and Fourier Transform Infrared (FTIR) spectroscopy
systems have been used as nondestructive methods that have the potential to test
honey purity. Fluorescence spectroscopy system consists of 405 nm diode laser,
mirror, ND filter and USB 2000+ Ocean Optics spectrometer, while FTIR
spectroscopy system consists of FTIR Prestige 21 / FTIR 8400 spectrometer.
Fluorescence spectroscopy was used to determine the relation between
fluorescence intensity to the dominant fluorescence wavelengths. FTIR
spectroscopy was used to analyze the spectra produced in accordance with peaks
formed from the results of sample measurements. Samples consisted of 6 forest
honey and 4 branded honey (BPOM certified, MUI and DEPKES permit), 1 date
syrup, 1 corn syrup and 4 honeys that mixed with corn syrup. Honey and nonhoney
samples were recorded using a USB 2000+ Ocean Optics spectrometer.
The values of sugar contents were measured manually using a refractometer brix.
The values of the dominant fluorescence wavelengths and the values of sugar
contents were analyzed. The result showed that the highest fluorescence intensity
is 1016.92 a.u at wavelength of 515.08 nm, while the lowest fluorescence
intensity is 185.31 a.u at wavelength of 641.30 nm that indicated by a non honey
sample. The results showed that the spectrum of pure honey samples peaked at an
area with wavelength of 500 nm. The difference of FTIR spectrum shape is shown
by the honey samples which is not given an adulteration, where the difference
begins to be seen clearly at the wave number range 1150 cm-1 to 650 cm-1.
Keywords: fluorescence spectroscopy, FTIR spectroscopy, fluorescence
wavelength, wave number, honey
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