CD Skripsi
Produksi Enzim Selulase Dan Xilanase Dari Eupenicillium Javanicum Dengan Substrat Bonggol Nanas Menggunakan Solid-State Fermentation
ABSTRACT
Bioprocess is a biotechnology science that utilizes microorganisms to
produce a biochemical product, one of which is an enzyme that can function as a
catalyst. Enzyme requirements continue to increase along with the growth of the
bioprocess industry. Cellulase and xylanase are enzyme products commonly used
in industry, such as pulp and paper, detergents, food, textiles, and others.
Pineapple tuber waste contains cellulose and hemicellulose which have potential
as pure substrates such as cellulose and xylan to produce cellulase and xylanase
enzymes. The process of enzyme formation can be done by the fermentation
process. This study aims to determine the effect of temperature (25, 30 and 35 °
C) and pH (4, 6 and 8) on the production of cellulase and xylanase enzymes in a
state of solid fermentation (solid-state fermentation) and compare the activity of
its enzymes under submerged fermentation. fermentation). The fermentation
process is carried out for 96 hours with the specified temperature and pH
conditions. Enzyme activity was measured using UV-Vis Spectrophotometry with
the DNS method at a wavelength of 540 nm. The optimum conditions obtained
were at a temperature of 35 ˚C and pH 6 with enzyme activity that is 0.262 U / mL
for cellulase enzymes and 1.683 U / mL for xylanase enzymes.
Keywords: Celullase, Enzyme, Solid-state fermentation, Submerged fermentation,
Xylanase
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