CD Skripsi
Isolasi Dan Identifikasi Bakteri Penghasil Biosurfaktan Asal Limbah Cair Perbengkelan
Abstract
Workshop activities produce oil spills that may pollute waters around the
workshop area. The oil polluted water may inhabit by biosurfactant producing
bacteria that is able to degrade the oil. A study aims to isolate and identify the
bacteria has been conducted on July to September 2020. The bacteria samples
were sampled from workshop wastewater at Kubang Raya street KM 2,5
Pekanbaru, Riau Province and sampling were conducted three times. The bacteria
were isolated using TSB (Tryptone Soy Broth) and TSA (Tryptone Soy Agar)
media, and were identified by using biochemical methods. Results shown that
there were seven types of bacteria, namely Providencia, Proteus, Acynotobacter,
Bacillus, Aeromonas, Proteus and Serratia. The Emulsification index of
Providencia was 38.8%, Proteus was 50%, Acinetobacter was 48.8%, Bacillus
was 52.1%, Aeromonas was 47.6%, Proteus was 54.7% and Serratia was 48.8%.
The bacteria isolates that produced the highest Emulsification Index was
identified using a PCR 16S rRNA and it is proved that the species is Proteus
mirabilis. Data obtained from this study shown that all of identified bacteria are
able to produce biosurfactants.
Keywords: Oil pollutant, Proteus mirabilis, Bakteria isolation, Mikroorganisms,
Emulsification Index.
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