CD Skripsi

Isolasi Dan Uji Penghambatan Α-Glukosidase Senyawa Metabolit Sekunder Dari Ekstrak Paku Kelakai (Stenochlaena Palustris)

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Stenochlaena palustris is one of the ferns that are often found in peat, and it has
medicinal properties including fever, immunostimulant, diarrhea, and antiaging.
According to a published paper, water extract from this species posses
α-glucosidase inhibitor. However, the secondary metabolites underliying this
activity has not yet been identified. Thus the objectives of this study were to
determine the potential the activity α-glucosidase inhibitor from various extract
and to isolate their secondary metabolites from the species. Air-dried aerial parts
of the species were extracted by using maceration in metanol and followed by
liquid-liquid extraction against n-hexane, dichloromethane (DCM) and ethyl
acetate to obtain n-hexane, dichloromethane, ethyl acetate and water extracts. The
α-glucosidase inhibitor activity of these extract revelead that DCM extract possess
the activity with IC50 value 406.26 μg/mL compared to n-hexane extract, ethyl
acetate and water. This extract is then purified through the chromatography
technique Vacum Liquid Chromatography (VLC) and yielded thirteen fractions.
In fraction 8 found solids that have the potential to produce crystals so it is
necessary to recrystallize to obtain crystals. The crystals (19-04-B-F8) obtained
had a melting point of 172-173 OC and phytochemical analysis revealed that the
compounds obtained belonged to the flavonoid group. Fourier-tranform infrared
spectroscopy (FTIR) analysis depicted the presence of absorption band OH at
3226 cm-1, absorption band C-H alkene at 3026 cm-1, absorption band C-H alkane
at 2890 cm-1, absorption band OCH3 at 2814 cm-1, absorption band C=O at 1696
cm-1, absorption band C=C at 1603 cm-1, absorption band C-H alkane at 1362
cm-1, absorption band C-O at 1261 cm-1, 1205 cm-1, 1179 cm-1, 1171 cm-1, 1085
cm-1, dan 1063 cm-1 dan absorption band C-H aromatic at 827 cm-1. The fraction
and the compound exhibited a range of inhibitory activities against α-glucosidase
with the compound (19-04-B-F8) exhibiting an IC50 value of 301.15 μg/mL.
Compound 19-04-B-F8 requires additional spectroscopic analysis and purification
of the active in order to establish it as a α-glucosidase inhibitor.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 02. 122 (0001)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2022

Deskripsi Fisik

x, 25 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

-

Klasifikasi

03 02. 122 (0001)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

-

Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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