SUMMARY Pulai basung (Alstonia spatulata Bl.) is one of medicinal plants from Alstonia genus and Apocynaceae family. The root, stem bark, leaves and fruits of this genus had been used in traditional medicine to alleviate some diseases. The goals of this study were to isolate and characterize secondary metabolites from chloroform fraction at pH 12 of stem bark pulai basung and analyze their tox…