Laccase (E.C1.10.3.2 : oxygen oxidoreductase) is a multi-copper enzyme that catalyzes oxidation process or release of substrate electrons using oxygen molecules as electron acceptors. Laccase used in this study came from Trichoderma asperellum LBKURCC1. The purpose of this study was to analyze the effect of metal ions (Cu2+, Co2+, Zn2+, Mn2+, Mg2+, and Fe2+) on the activity of laccase A and B, …