CD Skripsi
Potensi Bakteri Asam Laktat Dari Fermentasi Naniura Ikan Nila (Oreochromis Niloticus) Sebagai Penghasil Eksopolisakarida
Tilapia is a freshwater fish that is widely cultivated in Indonesia, especially in the city of Pekanbaru, and can be processed into economically valuable products such as naniura fermented products. The naniura fermentation process produces Lactic Acid Bacteria (LAB), which has the potential to produce secondary metabolites, namely exopolysaccharides (EPS). This study aimed to 1) To determine the effect of the duration of rough lemon (Citrus jambhiri) immersion on naniuraquality, Awvalue,pHvalue, total LAB, crudeEPScontent,2) To determine the characteristics of LAB from naniura LAB isolates of tilapia 3) To determine the best treatment for the duration of rough lemon immersion on naniura based on exopolysaccharide content. The research procedure consisted of three stages: 1) Preparation of naniura from tilapia with varying immersion times, 2) Isolation of lactic acid bacteria, and 3) Testing of crude exopolysaccharide production. The results showed the duration of rough lemon immersion had a significant effect on the organoleptic quality of naniura, Aw value, pH value, total LAB, and crude EPS content, 12 LAB isolates naniura belonged to the genus Lactobacillus with macroscopic characteristics, namely white and cream-colored colonies, flat edges, convex elevation, and round colonies. Microscopically, they were rod-shaped and gram-positive. Biochemical test resultsshowthat they werecatalase-negative, non-motile, and belong to the heterofermentative fermentation type, An immersion time of 5 hours was the best treatment with an EPS level of 3,864 mg/L.
Keywords: exopolysaccharide, fermentation, LAB, naniura, tilapia
1 Student, Faculty of Fisheries and Marine Science, Universitas Riau 2 Lecturer, Faculty of Fisheries and Marine Science, Universitas Riau
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