CD Skripsi
Uji Aktivitas Antimikrob Isolat Bakteri Asam Laktat Dadih Dari Kecamatan Limau Manis Kabupaten Kampar Riau Terhadap Bakteri Patogen
ABSTRACT
Dadih is buffalo milk fermented with lactic acid bacteria (LAB). This study
aimed to measure the antimicrobial activity of LAB isolates from dadih, Kampar
Regency, against the pathogenic bacteria Listeria monocytogenes, Staphylococcus
aureus, Salmonella sp., and Escherichia coli. This study was conducted
descriptively with four isolates and four replicates. Four LAB isolates (LM 1.1, LM
1.8, LM 1.9, and LM 2.4) with viability of more than 50% were tested for their
tolerance to gastric acid (pH 2), pepsin, and bile salts. The parameters observed
included the antimicrobial activity of LAB isolates and cell-free supernatants (CFS)
against Gram-positive (L. monocytogenes and S. aureus) and Gram-negative
(Salmonella sp. and E. coli) pathogenic bacteria. Data obtained from observations
of the antimicrobial activity of LAB isolates and CFS against pathogenic bacteria
in the form of clear zone diameters are presented in tables. The diameter of the clear
zone was analyzed descriptively by measuring the average and standard deviation.
The highest antimicrobial activity of LAB isolates against L. monocytogenes was
LM 1.9 and LM 2.4 at 10.83 mm, against S. aureus was LM 1.1 at 8.58 mm, against
Salmonella sp. was LM 1.9 at 8.33 mm, and against E. coli was LM 2.4 at 4.17 mm.
The highest antimicrobial activity of the cell-free supernatant against L.
monocytogenes was LM 1.9 at 16.25 mm, against S. aureus was LM 1.1 at 12.25
mm, against Salmonella sp. was LM 1.9 at 11.08 mm, and against E. coli was LM
1.9 at 6.42 mm.
Keywords: Antimicrobial activity, dadih, lactic acid bacteria, pathogenic bacteria
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